A physician, advanced practice registered nurse (APRN), or physician’s assistant (PA) performs a lumbar puncture.
A registered nurse (RN) or licensed practical nurse (LPN) is present during the procedure to assist in positioning the child and in assessing the child’s status.
The procedure is performed in a treatment room, unless the child is in an emergency room or intensive care unit and cannot be moved from the bed because of his or her condition.
Informed consent must be obtained before the procedure.
The procedure is carried out under aseptic technique.
Sterile disposable pediatric lumbar puncture tray to include:
Sterile drapes
A 3-mL syringe with 25-gauge needle
Three-way stopcock
Adhesive bandage
Three to five fluid collection tubes
Povidone-iodine swabs
Spinal fluid pressure manometer (optional)
Two lumbar puncture needles
Ampule of 1% lidocaine
Sterile gloves
Labels with child’s name for specimen tubes
Anesthetic agent (optional)
Assess the pertinent history and physical findings for signs of increased intracranial pressure.
Assess the age, developmental level, and coping strategies of the child.
Discuss with the child and family distraction techniques that may be useful during the procedure (e.g., imagery, focused breathing).
Assess the child’s and the family’s knowledge of the reasons for the procedure, expected outcomes, and potential adverse reactions.
Discuss role of the family member to provide support to the child if the family member will be present during the procedure.
Ensure that written consent has been obtained to perform the procedure.
Apply topical anesthetic to lumbar puncture site if using agent that requires advanced time for complete activation.
PROCEDUREAssisting With Lumbar Puncture
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Modification of the positions may be necessary if the child is intubated or has fractures.

KidKare A pillow or large stuffed animal can be placed in front of the chest with the child instructed to grasp it.
As of 2008 all chlorhexidine-based topical skin antiseptics have the warning “do not use for lumbar puncture or in contact with the meninges,” thus povidone-iodine antiseptic solutions are recommended to cleanse the skin before lumbar puncture.